Alzheimer's- Borrelia Infections

$2,184 of $25,000 goal

Raised by 17 people in 27 months
What did the Blood Antibody Test Show?
The answer to this single question determines
the eligibility  for "proper "diagnosis of human patients who may in fact be suffering from Tick Borne Borrelia Diseases.

Borrelia - Antibody - Negative - Research [BANR]
is dedicated to   new and cutting edge 21st century methods which will improve upon traditional  20th century blood testing which attempts to detect  antibodies to Borrelia spirochetal tick transmitted infections.  Lyme Serology is a medical word to describe 20th century methods to find Blood antibodies to  Borrelia infections.

21st century diagnostic methods are DNA based [BANR] and represent considerable improvements over the past reliance on blood antibody measurements. The DNA of the borrelia family of infections [BANR]-  the target here ( plural targets for plural Borrelia species.)

Antibody status is immaterial in DNA detection [BANR] procedures . Borrelia spirochetes which bind to  Species specific DNA probes, directly visualize under the microscope, the borrelia spirochetes in the body of the 
[BANR]  patient.

This  {BANR} funding appeal requests the support of persons who support  research which applies DNA based tools for identification of  Tick Borne infections in the human host.  The DNA  technology and the knowedge to apply 21st century molecular methods  are rapidly expanding into medical care of patients whose illnesses are difficult to diagnose.[BANR]

Funds donated to this outreach  BANR will be used to add to existing Validated Molecular DNA Based Methods [VMDBM} to be freely available to all physicians solely  for use in their  own medical offices. Free Training of Physicians who are motivated to learn how {VMDBM}  perform their own research  procedures in their own patient population, has already commenced. The Goal is  Tuition Free Education [TFE] of an international group of physician researchers to expand the use of DNA assisted evaluation their patient population for possible Borrelia related health issues.

The results of the  TFE research studies here,  will be placed in the public domain, and will never be patented or sold for profit. Patients under the care of  TFE graduates of this educational activity will be afforded the opportunity to submit tissues to a Research Borrelia Physician Consortium { RBPC]  for consensus evaluations.  Combined intelligence from a large group , RMPC of Physician researchers will be freely completed and sent to the  patient's physician of record for the individual patient.

The equipment needed for each physician performing such  testing  after graduation from the tutorial is
inexpensive and straightforward. It consists of  a microscope.  

The manufacture of  DNA tools to specifically bind to the DNA of Borrelia which cause human disease :  this cost  is presently about $1000.00 per probe and is supported by voluntary donations. One probe detects One species of borrelia. In the USA ,today, more than 100 subspecies of borrelia burgdorferi [Bb] and additional Miyamotoi Borrelia {MB]  species are now held in collections of the scientists who seek to develop better test methods. Each of the 100+ species
[MB + Bb}; will get its own DNA probe. Collectively,  in a mixture, this collection of yet to be manufatured Borrelia species DNAprobes will be combined in a single drop-to  fabricate a "Super-borrelia-DNA -probe" mixture. These Research Tools  will enable  Graduate practicing physicians the  exclusive use of the "widest possible detection net" to entrap the agents of borrelia infection- in their own physician office laboratories.

Point of Care Methologies are now routinely available
in many physician offices, and this campaign will expand the capabilities of individual physicians to, with a microscope in their private offices , to  evaluate their own patients for DNA evidence of borrelia infections.  This harkens back to the early 20th century, when every physician was trained in and equipped with a microscope and  with the necessary  reagents for staining bacteria  ( Gram Staining) , to assist in diagnosis of  infections their own patients.

All of this  Training and Technology is , with your support, freely given, and freely received ; a high technology gift to physicians in practice for the unique benefit of their patients.  

Your financial gifts to support this unique incentive to improve the quality of patient care are gratefully received.

Please Donate to this outreach,  and please know, that
no donation is too small to advance better patient care
in borrelia related human illnesses.

Respectfully,
Alan B. MacDonald, MD
November 20,2015
+ Read More

Do Infections Cause Alzheimer’s Disease?
Biofilms
The hippocampus of an Alzheimer’s disease patient brain showing the presence of what are believed to be biofilms — assemblages of bacteria that exude slimy, glue-like substances that can tolerate antibiotics.

A study from Drexel researchers offers new evidence to support a controversial hypothesis: Alzheimer’s disease results from the body’s inflammatory response to chronic infections. The scientists believe that bacteria create slimy, impenetrable biofilms in the brain, prompting the immune system to destroy surrounding tissue. This intriguing idea — though supported by a growing body of documentation — is still contested by many in the scientific community.
The disease is characterized by plaques in the brain, which form when sticky protein pieces called beta-amyloid clump together. Despite billions being invested to understand how the beta-amyloid contributes to a slow, cognitive decline, the disease remains a mystery.
“We have done absolutely nothing to change the course of the disease. Other chronic diseases, we have affected, because we understand their etiology — what causes them,” says Garth Ehrlich, PhD, a professor in the College of Medicine and director of the Center for Genomic Sciences. “There is nothing new you can do for an Alzheimer’s patient that you couldn’t do 20 years ago.”
Dr. Ehrlich and other Drexel faculty members are among a group of scientists who think the direction of Alzheimer’s research needs to be rerouted.
“The lack of progress in Alzheimer’s research suggests that the etiological hypotheses followed by the mainstream scientific community are either wrong or incomplete,” Ehrlich says.
A Novel Theory
In the early ’90s, pathologist Alan MacDonald theorized that Alzheimer’s disease may have a bacterial component. Later, Swiss researcher Judith Miklossy made a profound observation when she showed that neurosyphilis (a bacterial disease of the brain) and Alzheimer’s were essentially identical.

Herbert Allen, MD, chairman of Drexel’s Department of Dermatology, who has treated patients with both Lyme disease and syphilis.:
biofilms — multicellular assemblages of bacteria that exude slimy, glue-like substances. Once bacteria metamorphose into a biofilm, antibiotics can no longer treat them. The 2014 study proposed that staphylococcal bacteria that live on the skin produce a biofilm that blocks sweat ducts, activating the immune system
Allen’s newest study, published this week in Neuroinfectious Diseases, suggests that spirochetes in the brain could be operating in the same way to cause Alzheimer’s.
Allen and a team of scientists investigated seven post-mortem brains of Alzheimer’s disease patients, comparing them to 10 control samples from people who had died from other causes.
In each of the Alzheimer’s brains, the researchers saw what they believe to be biofilms. The researchers also identified a protein called TLR 2, which plays a key role in activating the body’s innate immune system.
“So it’s neither the infection, nor the biofilm, nor the beta amyloid that causes the disease, but the body’s own immune system,” Allen says.
As for why Alzheimer’s primarily strikes older adults, the study’s authors note that spirochetes take 90 days to divide, so the process takes a long time to build up sufficient numbers of organisms to form a biofilm. However, after someone suffers a stroke, the adaptive immune system — which the authors liken to a “machine gun” — is activated and eradicates organisms with much greater efficiency than the innate immune system.
“However, just as in the situation with the innate immune system, it cannot penetrate the biofilm either, thus it destroys greater amounts of the surrounding neural tissue much more rapidly,” the researchers write.
The hypothesis could explain why symptoms of dementia can appear suddenly after a stroke, rather than slowly over time, as is observed with traditional Alzheimer’s patients.
A Paradigm Shift
Further research will be needed to confirm that spirochetal bacteria (or any bacteria for that matter) can trigger the inflammatory cascade that leads to Alzheimer’s disease. Some experts note that the sticky substance seen in the brain tissue sections from Allen’s study could actually be the beta-amyloid, rather than the presence of biofilms.
However, Allen’s observations add fuel to a growing body of data that makes the connection between bacteria and Alzheimer’s.
Ehrlich (who was unaffiliated with Allen’s study), is one of the world’s leading experts in biofilms. He has dedicated his career to trying to understand how bacteria cause chronic disease, and he agrees that testing the hypothesis that biofilms play a triggering role in Alzheimer’s is a worthwhile pursuit.
In his lab, he is conducting a blinded study of brains from both Alzheimer’s patients and controls in which multiple DNA tests are being used to first identify and then confirm the presence of any bacteria. The researchers will then determine which genes the bacteria are expressing by performing species-specific RNA analyses.
If their hypothesis is confirmed, the findings could shift the paradigm of Alzheimer’s disease research. Rather than developing drugs to try and target the beta-amyloid (as is the direction of most current studies), researchers could be putting efforts into developing more reliable tests for bacterial detection in the central nervous system and investigating new anti-biofilm treatments.appreciative of the “bacterial” Alzheimer’s hypothesis,
Ehrlich defers to a quote from German physicist Max Planck: “Science advances one funeral at a time.”

“Scientists are supposed to be the most open-minded people in the world, but many of them are not,” Ehrlich says. “From my perspective, people should go into science to be very open-minded and to test any valid hypothesis. And certainly not stand in the way of people who want to test something you don’t think is right.”
Media interested in talking with Ehrlich or Allen should contact Lauren Ingeno .
ALZHEIMER'S PLAQUES ARE BIOFILMS
+ Read More
Update : November 26, 2015
FISH method using Molecular Beacon DNA probes
to directly visualize with a microscope, Disease producing Borrelia in Body tissues.
In this case a 19 month old male died( unknown causes )and a Medical Examiner Autopsy was performed.
At the request of the parents, glass slides from the medical examiner's office were sent to me from the autopsy heart and autopsy brain.
FISH methods demonstrated an overwhelming number of Borrelia spirochetes in both Heart and in Brain.

Link:
https://www.dropbox.com/s/xzqqzvk47sr7fpt/SK%20Autopsy%20Brain%20and%20Heart%20with%20Beacons%20for%20B%20Burgdorferi.pdf?dl=0

Conclusion: Cause of Death: Overwhelming Borrelia infection, not considered by the physicians caring for the child.
Borrelia spirochetes in Heart
Borrelia spirochetes in heart by FISH
Biofilm of borrelia in heart by FISH
+ Read More
Update: Norwegian Spinal Fluid Volunteer-
Positive results for two strains of Borrelia
with DNa probes
Patient , adult, male, with Multiple Sclerosis
Borrelia Antibody Tests- NEGATIVE
DNA probe method - Diagnostic of Borrelia
infection in Spinal Fluid
--
Physician of Record: Joe Siri Ekgren MD
Report Date: May 1, 2015
Specimen: Air Dried Cerebrospinal fluid Films on Glass Slides
Fluorescence In situ DNA Hybridization by Alan B. MacDonald MD
Controls: All Positive and Negative controls Acceptable
Specimen Quality: Optimal Monolayer Film preparations for FISH study
Reagents: All Molecular Beacon DNA probes: BBO 0147 Burgdorferi group Flagellin B DNA Probe,(FITC)
BBO 0740 Burgdorferi Group sl (including European Borrelia) Inner cell membrane DNA probe (FITC)
Miyamotoi Borrelia Flagellin B DNA Probe ( Cy6 Fluorochrome-red), Miyamotoi Borrelia glpQ Dna Probe
(Cy3 Fluorochrome – yellow)
RESULTS:
1. Positive Detection of Borrelia Burgdorferi group sl DNA and spirochetes Imaged directly
With DNA probes. [ Biofilm Communities detected in specimens provided}
[ Biofilm communities Always indicate a Chronic Borrelia Infection]
2. Positive Detection of Miyamotoi borrelia sl DNA and spirochetes imaged directly
With DNa probes. .
NOTE: !!! _ Detection of Biofilm Communities of Borrelia Miyamotoi in
Cerebrospinal fluid - the first of its kind discovery of
Spinal fluid borrelia biofilms.!!




Images presented on next pages


FISH detection of Burgdorferi group sl
Spirochete with DNA probe BBO 0147
( Flagellin B)




Fish Detection of Burgdorferi Group sl spirochetes in a Biofilm Community with
DNA probe BBO 0740 ( Inner Cell Membrane)



Fish Detection of Burgdorferi Group sl spirochetes in a Biofilm Community with
DNA probe BBO 0740 ( Inner Cell Membrane)




FISH detection of Miyamotoi Group sl borrelia
Spirochetes with DNA probe for Miyamotoi Flagellin B DNA




FISH detection of Miyamotoi Group sl borrelia
Spirochetes with DNA probe for Miyamotoi Flagellin B DNA

FISH detection of Miyamotoi Group sl borrelia
Spirochetes with DNA probe for Miyamotoi
glpQ DNA




Diagnosis: and Remarks:

Two strains of Borrelia spirochetes recovered by FISH Method from Spinal Fluid
Of an Anonymous Volunteer resident of Norway
With Multiple Sclerosis

1. Burgdorferi sl group includes all European strains of Borrelia
2. Miyamotoi sl group includes all
Currently known strains of
Miyamotoi borrelia.
( Note the glp Q marker is never present
In burgdorferi sl Borrelia. It is present in Miyamotoi borrelia. The Actual glpQ
DNA probe sequence differs slightly
(Continuation)

From the glpQ DNA Sequence of
Other Relapsing Fever Group Borrelia.
The glpQ DNA probe used here is
Unique to the Miyamotoi DNA for
The Glp Q locus.


Miyamotoi sl group borrelia are
Vectored by hard ticks.
Reservoirs of infection with Miyamotoi
Have been documented in Scandinavia.


Respectfully submitted,
Alan B. MacDonald, MD
Fellow, College of American Pathologists
Borrelia burgdorferi in spinal fluid
Miyamotoi Borrelia in Spinal fluid
Miyamotoi Borrelia in Spinal fluid
+ Read More
Read a Previous Update
Jack Dupre Stravinsky
27 months ago
2
2

Kathleen Dickson do not get caught up in OspA presence in vaccines, as the vaccines could have easily been contaminated with Borrelia blebs (aka spores, aka granular forms) or even Borrelia cysts. Borrelia in those morphologies (typically 1 micron on less) could have easily escaped attenuation of the vaccines & would result in those forms maturing into full blown spirochetes & full blown Lyme Disease. Please do your homework & do it outside the confines of the narrow minded CDC morons!!!

+ Read More
Kathleen Dickson
27 months ago
1
1

There already is an FDA-validated test for Lyme, US patent 5,618,533, developed by Fikrig and Flavell in 1991, Remember I did this for a living at Pfizer - develop and FDA-validate analytical methods. Band 41 was made SPECIFIC. The problem is that late in the disease or neuroLyme is not about spirochetes if the OspA vaccine caused the same systemic disease. Please do your homework http;//www.actionlyme.org

+ Read More
Thomas Grier
27 months ago

It is important to note that this patient had extensive and prolonged antibiotic treatment, yet the Borrelia were still present and still active in several sequestered spaces. The human testicle is an immune privileged site and white blood cells don't easily enter the testicular tissue. So once a Borrelia infection takes hold within the testes, it has few natural enemies, and the lower temperature is ideal for Borrelia burgdorferi to thrive.

+ Read More
Brooke Kramer
4 months ago

You guys are probably all right about this in some way or the other, absolutely CRUCIAL not to have inter Lyme community disagreements about the specifics PARALYZE the political action arm for research money, care or justice for perps. Lyme is so complex. I had 1000 mg abx and did nothing to erase over time. It has spread involve a rare mitochondrial disease and signs of compromised immune function, particularly involving cytokines and low angiogrowth and now nervous dysfunction. I am having more tests and may soon find there is no longer even active borrelia growth at least in the blood. Obviously, this illness knocks down immune function involving macrophages and the adaptive immune system (b cells) as well as activating t killer cells in some of way that can't be tracked on tests. I am sure you are all experts and can tell me more. I'm really concerned about the fate of the current effort to get a congressional investigation on ALDF and IDSA. The blackops government connections are so strong and the various cults so cross tied to shut people down I can easily imagine the gatekeepers on this effort of whatever committee is formed will only serve to shut the whole thing down before it even reaches the House of Representatives. Democracy is completely gone in America. We are all under the surveil of powerful agencies of the deep state with ties all over in gatekeeper positions, even the Congress, courts, president are clearly dancing bears.

+ Read More

$2,184 of $25,000 goal

Raised by 17 people in 27 months
Created November 20, 2015
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SM
$20
Sebastien Millon
22 months ago

keep up the great work Dr MacDonald! hope you are doing well :)

MM
$500
Monique Millon
24 months ago

We have to get these diseases- Lyme and Co-infections really taken care of. Dr. Steer and his friends and colleagues have done too much harm to a whole generation of young people who have seen their lives passing by them and have been in terrible pain.....criminals is what I would call these doctors who have forgotten they should do no harm!

$25
Anonymous
24 months ago
VM
$100
Virginia Sherr, MD
27 months ago

Thank you, Dr. MacDonald, for once again sharing your research to further the meaningfulness of the illnesses and/or deaths of the innocents about whom you care so much --whether or not such sad losses relate to the passing of your colleagues in research or to a tiny babe, you find a way to make such losses visible and thus priceless to all others who suffer from the scourge of tick-borne illnesses, especially Lyme disease. It is a privilege to be able to join you in this campaign to honor those who can not speak for themselves. Ginny Sherr, MD The Human Side of Lyme/an inhuman brain disease

RA
$48
Ross Anderson
27 months ago

Its Giving Tuesday. I'm Thankful for your work Dr. MacDonald.

Jack Dupre Stravinsky
27 months ago
2
2

Kathleen Dickson do not get caught up in OspA presence in vaccines, as the vaccines could have easily been contaminated with Borrelia blebs (aka spores, aka granular forms) or even Borrelia cysts. Borrelia in those morphologies (typically 1 micron on less) could have easily escaped attenuation of the vaccines & would result in those forms maturing into full blown spirochetes & full blown Lyme Disease. Please do your homework & do it outside the confines of the narrow minded CDC morons!!!

+ Read More
Kathleen Dickson
27 months ago
1
1

There already is an FDA-validated test for Lyme, US patent 5,618,533, developed by Fikrig and Flavell in 1991, Remember I did this for a living at Pfizer - develop and FDA-validate analytical methods. Band 41 was made SPECIFIC. The problem is that late in the disease or neuroLyme is not about spirochetes if the OspA vaccine caused the same systemic disease. Please do your homework http;//www.actionlyme.org

+ Read More
Thomas Grier
27 months ago

It is important to note that this patient had extensive and prolonged antibiotic treatment, yet the Borrelia were still present and still active in several sequestered spaces. The human testicle is an immune privileged site and white blood cells don't easily enter the testicular tissue. So once a Borrelia infection takes hold within the testes, it has few natural enemies, and the lower temperature is ideal for Borrelia burgdorferi to thrive.

+ Read More
Brooke Kramer
4 months ago

You guys are probably all right about this in some way or the other, absolutely CRUCIAL not to have inter Lyme community disagreements about the specifics PARALYZE the political action arm for research money, care or justice for perps. Lyme is so complex. I had 1000 mg abx and did nothing to erase over time. It has spread involve a rare mitochondrial disease and signs of compromised immune function, particularly involving cytokines and low angiogrowth and now nervous dysfunction. I am having more tests and may soon find there is no longer even active borrelia growth at least in the blood. Obviously, this illness knocks down immune function involving macrophages and the adaptive immune system (b cells) as well as activating t killer cells in some of way that can't be tracked on tests. I am sure you are all experts and can tell me more. I'm really concerned about the fate of the current effort to get a congressional investigation on ALDF and IDSA. The blackops government connections are so strong and the various cults so cross tied to shut people down I can easily imagine the gatekeepers on this effort of whatever committee is formed will only serve to shut the whole thing down before it even reaches the House of Representatives. Democracy is completely gone in America. We are all under the surveil of powerful agencies of the deep state with ties all over in gatekeeper positions, even the Congress, courts, president are clearly dancing bears.

+ Read More
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