We are looking for additional funding to make more biological materials available to researchers and lab worldwide to advance research.
The freegenes “store” is available at stanford.freegenes.org . With the current pandemic we are working with researchers to distribute COVIID-19 related materials:
COVID-19 Diagnostic Toolkit Enzymes
A collection of expression vectors for un-patented or off-patent enzymes used in diagnostic tests for COVID-19. It includes HIV reverse transcriptase, MMLV reverse transcriptase, Bst-LF polymerase, RNAse inhibitor and Taq Polymerase. Attached are documentation on each gene and its expression.
COVID-19 Mammalian ORFs and Expression Vectors
A collection of COVID-19 ORFs optimized for Mammalian expression. They come in two different formats, in expression vectors and in gateway compatible vectors.
In conjunction with Jenny Molloy of the University of Cambridge and The Open Bioeconomy Lab we are distributing to laboratories around the world and partner labs in Africa:
Open Enzyme Collection
The Open Enzyme Collection consists of essential enzymes that are workhorses of molecular biology and are commonly used in labs for techniques involving detecting, altering and manipulating DNA. The collection is codon-optimised for expression in Escherichia coli bacteria and all enzymes were obtained from expired patents or for various reasons are not encumbered by patent rights. We have also produced special collections of Open Reporters, Regulatory Elements and a Research in Diagnostics Collection tailored to the needs of groups developing molecular diagnostics.
Enzymes are the heart of each and every diagnostic test. Enzymes are the means we can track, study and defeat old and new infectious diseases. However, enzymes are globally distributed from centralized foundries and, most often, require a cold chain for stability restricting their access to the many, even more in times of supply chain disruptions like is happening in the current COVID-19 pandemic.